HeLa (cervix adenocarcinoma from American Type Culture Collection, VA, USA)
treatment
None
passages
13-15
chip antibody
A305-096A
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were then crosslinked, collected and lysed according to the "ChIP-IT High Sensitivity" kit (Active Motif). Sheared chromatin was obtained using a probe sonicator and used for ChIP experiments using RNAPII and YTHDC1 antibodies. ChIP-seq librairies were constructed using the Next Gen DNA Library Kit (Active Motif 53216 and 53264).